Fatty acids are crucial in order to obtain optimal peak bone mass. The composition of fatty acids in 8-month-old, healthy silver foxes and their interrelationships with the morphological, densitometric, and mechanical properties of the mandible and bone metabolism markers was studied. We used gas chromatography and the ELISA and EIA methods to evaluate the biochemical profile of the serum. Quantitative computed tomography, dual-energy X-ray absorptiometry, and the three-point bending test were used for mandible evaluation. In males, elaidic acid content was lower by 3.21%, while oleic acid content was higher by 4.87% when compared to females (p<0.05). With the exception of stearic acid found in saturated fatty acids, the concentrations of caprylic, capric, palmitic, and heptadecanoic acids and the total sum of saturated fatty acids have shown only negative correlations with bone formation markers, and the morphological and densitometric properties of the mandible. Monounsaturated fatty acids (heptadecenoic and eicosenoic acids) were both positively and negatively correlated with bone properties, while erucic, elaidic and oleic acids showed only positive correlations with bone traits and bone formation markers (p<0.05). Among the polyunsaturated fatty acids, linolenic and arachidonic acids were negatively correlated with densitometric parameters, while linoleic acid showed the highest number of positive correlations with densitometric and mechanical properties (p<0.05). Eighteen-carbon-chain fatty acids were mainly positively correlated with the morphological, densitometric and mechanical properties of the mandible. The most positive implications for bone metabolism and bone properties, in regard to fatty acids, may be ascribed to oleic and linoleic acids. The elaborated experimental model may serve for further studies on bone metabolism regulation in monogastric mammals using dietary modifications of fatty acid content.