The importance of research on honeybee queen immunity is underscored by the fact that it is the only reproductive caste in the bee colony, whose genetic and biological quality largely translates into the condition and productivity/efficiency of the colony. Queen bee fat body and tergal glands play crucial roles in bee immunity. The role of the fat body is not limited to storing energy reserves in the form of fat, protein, or glycogen. This tissue has detoxifying functions and participates in the maintenance of homeostasis. Additionally, the secretion of tergal glands allows workers to recognize the queen and is an attractant for drones during mating flights. The characteristics of tergal glands in queens are important for understanding the biology of reproduction. We determined changes in the morphological image of fat body and tergal gland cells in uninseminated Apis mellifera queen bees. We showed that the segmental character of the fat body depends on its location, not only in 1-day-old queens, but also in those at 8 and 20 days of age. The morphological image of tergal gland cells was not different in the queens aged 1 and 8 days, but dark-colored lumps appeared in the cells of those aged 20 days.