Mineral fertilisers are applied to the soil to replenish or maintain at the necessary level its abundance of available nutrients and to optimise the genetic production potential of plants. Humic substances are biologically active compounds of organic matter in the soil that effectively support mineral fertilisers in the cultivation of various crops. Therefore, the aim of this study was to determine the effect of increasing doses of humic acids-based fertilisers (HAs) on the regulation of macroelement contents in maize grown on two soils (loamy sand and sand) fertilised with different nitrogen fertilisers (ammonium nitrate - AN, urea - U and urea and ammonium nitrate solution - UAN). Nitrogen fertilisation had a greater effect on the macroelement content of plants on sand. The highest macroelement contents in maize grown on this soil was found on soils fertilised with UAN. Urea induced similar but smaller changes in the K, P and Na content of maize, but not in Mg and Ca. Urea also had the most favourable effect on plant biomass. HAs tended to increase the macroelement content of plants. On the sand, HAs had the greatest effect on the UAN fertilised objects, causing an increase in Mg, P, Ca and K compared to the unfertilised object. On loamy sand, the changes in their contents in maize were less clear. HAs caused an increase in P, K, Ca and Mg in the series with urea and Ca in the pots with ammonium nitrate, compared to the object without their application. They also caused a reduction in the P content in the ammonium nitrate pots and in the Na content of the maize in the UAN pots. HAs can be a good source of essential elements for plants by supporting the effectiveness of mineral fertilisation.