Background: The ecological approach to agriculture causes the desire to eliminate pesticides. Work is underway to replace them with natural agents. Aim: The aim of the research was to introduce an innovative factor and non-invasively increase the yield of dry matter and starch and thus improve the quality of the raw material for the production of potato-enriched products. Material and methods: The field experiment was carried out in central-eastern Poland, using the randomized sub-block method, in a dependent split-split-plot design in three repetitions. The superior factor of the first order was pre-planting treatments by treating seed potatoes with a microbiological preparation for (a) 10 min, (b) 15 min, and (c) untreated seed potatoes (untreated control). The second-order factor was 14 potato cultivars of all groups of earliness. Qualitative research was carried out under laboratory conditions. Results: Pre-planting treatments with the use of a microbiological preparation had a positive effect on the yield of dry matter and starch compared to the control while modifying the characteristics related to the quality of potato chips. The tested cultivars determined both the quantitative and qualitative characteristics of the chips to the greatest extent. Conclusions: Alternative solutions will allow the effective replacement of pesticides in potato production and processing.