Currently, one of the most sensitive indicators assessing the usable quality of soil is the assessment of enzymatic activity and total antioxidant capacity (TAC). They reflect, to a large extent, the possibility of producing high-quality plant materials, which is particularly important in protected areas, such as the buffer zone of a national park. The aim of the study was to assess catalase activity (CA) and antioxidant properties of brown soils depending on its use, located in the buffer zone ofthe Roztocze National Park. The material for the study consisted of soil samples collected from the humus horizon (A), browned horizon (Bw) and parent material (C) of three research plots.