The aim of the study was to determine the effect of Fe glycine chelate supplementation on the chemical composition and nutritional quality of breast meat from broiler chicken. The following parameters were assessed: fat content, cholesterol content, fatty acid profile, atherogenic index (AI), thrombogenic index (TI), and hypocholesterolemic/hypercholesterolemic (H/H) fatty acid ratio. The 42-day experiment involved 200 broiler chickens assigned into four dietary groups: the control receiving Fe sulfate in the dose of 40 mg/kg of feed and three experimental groups of chickens supplemented with 40 mg (Fe-Gly40), 20 mg (Fe-Gly20), or 10 mg (Fe-Gly10) of Fe glycine chelate per 1 kg of diet. The results showed no negative effect of the application of Fe glycine chelate on the chemical composition and nutritional quality of breast muscle. Therefore, the advisability of the application of Fe glycine chelates in the nutrition of broiler chickens should be revised.