The aim of this study was to test the usefulness of environmental enrichment for Eublepharis macularius depending on the maintenance method (terrarium vs. rack system). The hypothesis was that reptiles kept in an extremely low-stimulus environment (rack system) would be more prone to interact with environmental enrichment items than those kept in a biotope terrarium. During the study, 21 female geckos were kept in two types of captive enclosures: 9 in terrariums, and 12 in rack system boxes in groups of 3 animals per enclosure. During the enrichment sessions, geckos were observed for 45 min while enrichment items (dry and wet hides, a new feeding method, a new object) were present in the enclosure. All geckos showed interest in enrichment items that enabled hiding and climbing. Animals kept in the rack system showed significantly lower latency in approaching enrichment items and a higher frequency of enrichment interactions than lizards in biotope terrariums. However, no significant differences were found in the total time spent interacting with enrichment items between geckos in the two settings.