In the current study the hypothesis that 1.0% dietary inclusion of glutamine (Gln), a conditionally essential amino acid that influences protein synthesis and shows anti-osteoporotic effect, can influence eggshell quality was tested on laying hens in the post-peak laying peri-od. Thirty-week-old Bovans Brown hens were randomly assigned to control group or group supplemented with Gln in the form of alpha-ketoglutarate (10 g/kg) with 12 replicate cages (2 hens/cage) in each group. The experimental period lasted for 30 weeks, from the 31st to the 60th week of age of hens, when eggs were collected and selected eggshell quality indices were determined. While Gln supplementation had no effect on egg geometry and eggshell cracking force, the analysis of eggshell quality showed an increase of the eggshell thickness (P<0.01), porosity (P<0.01), eggshell specific surface (P<0.001) and positive changes in other mechanical parameters (decrease of eggshell stiffness and Young’s modulus, P<0.05 and P<0.01, respectively; increase of work needed to crack the eggshell, P<0.05). Changes in egg-shell mineral composition, including an increase of Ca content (P<0.001), were also observed. In conclusion, the current study showed a beneficial effect of Gln on the eggshell thickness, calcification, and some mechanical parameters