Physiological roles of red carrot methanolic extract and vitamin E to abrogate cadmium‐induced oxidative challenge and apoptosis in rat testes: involvement of the Bax/Bcl‐2 Ratio

Abstrakt

The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl2) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (n = 12) are used; control, CdCl2, CdCl2 + Vit. E and CdCl2 + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl2 on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl2 significantly succeeded in the modulation (p < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl2 insult.

Autorzy

Ahmed Abdel-Wahab
Ahmed Abdel-Wahab
Kamel M.A. Hassanin
Kamel M.A. Hassanin
Ahmed A. Mahmoud
Ahmed A. Mahmoud
Walaa I.E. Badeea
Walaa I.E. Badeea
Abdel-Razik Abdel-Razik
Abdel-Razik Abdel-Razik
Eman Zekry Attia
Eman Zekry Attia
Usama Ramadan Abdelmohsen
Usama Ramadan Abdelmohsen
Rabie L. Abdel Aziz
Rabie L. Abdel Aziz
Ibtesam S. Alanazi
Ibtesam S. Alanazi
Khalaf F. Alsharif
Khalaf F. Alsharif
Mohamed M. Abdel-Daim
Mohamed M. Abdel-Daim
Mohamed O. Mahmoud
Mohamed O. Mahmoud
artykuł
Antioxidants
Angielski
2021
10
11
1653
otwarte czasopismo
CC BY 4.0 Uznanie autorstwa 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2021-10-21
100
7,675
1
7