The quality and potability of waters of the Tyśmienica River Basin were determined in 2017 at eight measuring stations. The paper presents analyses of the physical and chemical parameters of surface waters of the basin. With regard to suspended solids, pH value, electric conductivity, sulphates, ammonia, chlorides and phosphate, the water was classified as having a very good ecological status. In the case of Kjeldahl nitrogen, the waters were classified as having a good ecological status. In the case of the remaining parameters, i.e. BOD, DO, TOC and COD, the status varied among stations. The values of all the physical and chemical parameters complied with the values for undisturbed conditions. Only ammonia and COD showed significant differences among stations. The WPI values for each measuring point ranged from 0.90 to 1.56, what corresponds to the descriptive indicators of moderately polluted water. The high concentrations of COD and TOC indicate that high-performance treatment processes (category A3) must be used to ensure that the water is suitable for drinking.