the aim of the study was to compare the content of selected bioactive whey proteins and lipophilic vitamins, which largely determine the bioactive status of milk, in milk obtained from local breeds of cows raised in poland (polish red, white-backed and simmental) and polish holstein-friesian cows. Basic chemical composition and content of selected bioactive compounds (β-lactoglobulin, α-lactalbumin, lactoferrin, bovine serum albumin, and vitamins: E, A, D3 and β-carotene) in 550 milk samples were analysed. the milk produced by local breeds proved to be a more valuable source of whey proteins and lipophilic vitamins than that of the polish holstein-friesians. it was distinguished by its content of all bioactive compounds, including those with antioxidant proper-ties, i.e. vitamins e, a and D3 and β-lactoglobulin, and antimicrobial properties, mainly lactoferrin. therefore, the milk of cows of local breeds should be considered a valuable material for process-ing, especially at the level of the farm or small local processing plant, to produce products that are richer in health-promoting compounds. the promotion of milk from local breeds can therefore be an opportunity for the farms that raise them.