Cisplatin (CPL) is a common antineoplastic drug used in human medicine for treatment of various cancer types. Since the knowledge about its effects on crustacean behavioral and physiological parameters is very scarce, the aim of our study was to determine the influence of CPL at concentrations of 125 μg/L, 200 μg/L, 500 μg/L and 1000 μg/L on swimming behavior (swimming speed, distance travelled, hopping frequency, propelling efficiency index - a novel parameter) and physiological parameters (heart rate, thoracic limb activity) of Daphnia magna with the use of video digital analysis. The results showed that distance travelled, swimming speed, hopping frequency and propelling efficiency were inhibited as early as after 24 h in concentration- and time-dependent manner. On the other hand, heart rate was stimulated in the animals treated with 125 μg/L of CPL after 48 h, 72 h and 120 h of the exposure, however it was decreased at the higher concentrations. Although thoracic limb activity was considerably increased in daphnids exposed to 125 μg/L and 200 μg/L after 72 h, it was inhibited at the higher concentrations of the drug. The study suggests that since CPL affected daphnid parameters at the environmental concentration, it should be considered as hazardous to zooplankton.