The aim of the study was to determine the degree of relatedness between individuals in the Polish Hunting Dog population and to assess the genetic variability of the population based on pedigree analysis and molecular DNA testing. Pedigree data on Polish Hunting Dogs provided by the Polish Kennel Club was used. In total, data on 2783 individuals were obtained. DNA was obtained from cheek swabs taken from 177 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat - STR) was used. On the basis of genotypes in microsatellite loci, assessment of the Hardy-Weinberg genetic equilibrium was also carried out in the analyzed dog population using the ?2 test. The pedigree data contributed to identification of 26 founders. The Founder Genome Equivalent was 4.17, Founder Genomes Surviving was 17.03 and effective population size (Mean Ne) was estimated at 28.51. The gene diversity was 0.8802, the population mean kinship was 0.1198, and the mean inbreeding was 0.1151. Studies of STR markers revealed polymorphic information content – PIC = 0.56, and the estimated values of expected heterozygosity were generally close to the observed heterozygosity values and on average amounted to 0.6050 and 0.6142, respectively. The calculated average inbreeding coefficient (FIS) value was negative -0.012, which suggests a low level of inbreeding in the examined breed as well as avoidance of related animals in mates.