This study evaluated the influence of extreme effort on physiological parameters of males and females with low or moderate levels of physical activity (PA) and different body composition parameters. Ninety-six participants (45 females, aged 20.05±1.81 years; 51 males, aged 20.20±2.71 years) took part in this study. PA levels were evaluated with the International Physical Activity Questionnaire (IPAQ) and they performed the 3-Minute Burpee Test (3-MBT). The Mann-Whitney U test and correlation analysis were applied. Participants with low levels of PA had significantly higher (p<0.05) body mass and body composition parameters (total body water, protein, minerals, body fat mass, fat-free mass, skeletal muscle mass, body mass index, percent body fat and waist-hip ratio (WHR). Participants with moderate levels of PA completed significantly more training cycles during the 3-MBT than their sedentary peers. Physiological parameters and exercise intensity were higher, albeit non-significantly, in more physically active participants. Extreme effort duration was significantly (p=0.0445) longer in males with low levels of PA (00:29 min) than moderate levels of PA (00:15 min). Females and males with moderate levels of PA have significantly higher endurance-strength abilities and are characterised by non-significantly higher values of physiological parameters during extreme effort than their sedentary peers.