We identified the beetle fauna of springs of a small lowland river and attempted to determine the directionand magnitude of beetle migration between the springs and neighboring water bodies in the river valley, as well as thelocal environmental factors and landscape parameters that most influence the character of aquatic beetle assemblagesin the springs. We studied springs of three limnological types, along the entire length of the river valley, and identified42 beetle species. All types of springs were dominated by stagnobiontic species, which enter springs from other aquaticenvironments, mainly via dispersion by air. We also found a small proportion of crenophiles and a substantial proportionof rheophiles and tyrphophiles, which was linked to the close proximity of the river and dystrophic water bodies. Thefauna of the springs was affected to a similar degree by local environmental factors and by landscape factors acting ona broader scale. This indicates the need for broader consideration of landscape factors, which are often neglected inecological studies.