The relation between neutrophil action, acute phase proteins, and ascorbic acid concentration in plasma of calves in the course of BRD (Zależność pomiędzy aktywnością neutrofili, poziomem białek ostrej fazy i stężeniem kwasu askorbinowego w osoczu krwi w przebiegu zespołu oddechowego u cieląt)

Abstrakt

Neutrophils, apart from their role in immunodefence, are also connected with lung injury, because of excessive release of enzymes such as elastase and myeloperoxidase (MPO), as well as nitric oxide (NO) generation. The purpose of this study was the relation between release of neutrophil products, acute phase proteins level (APP) and ascorbic acid (AA) concentration in the course of BRD in calves. The study was carried out on 10 healthy and 10 BRD calves. Neutrophil enzymes were assessed spectrophotometrically, NO level was determined by Griess reaction. Fibrinogen level was estimated by the heat precipitation method. Haptoglobin was assessed by the Owen’s method. AA analysis was done after reaction with dinitrophenyhydrazine. We estimated that elastase (71.97 ± 3.89%) and MPO (29.7 ± 5.88%) release from neutrophils isolated from BRD calves was significantly higher than from healthy animals (54.23 ± 5.66% and 19.18 ± 8.3%, respectively, p < 0.05). Moreover, our experiment revealed the positive correlation between secretory action of neutrophils and APP. We also observed that increased secretory action correlated negatively with plasma concentration of AA.

Autorzy

Marzena Olszewska
Marzena Olszewska
artykuł
Annales Universitatis Mariae Curie-Skłodowska Sectio DD, Medicina Veterinaria
Angielski
2009
64
1
7-14
otwarte czasopismo
CC BY-NC-ND Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 4.0
ostateczna wersja opublikowana
w momencie opublikowania
2009-03-31
2
0
0
0