The objective of the research was to evaluate the effect of recreational and hippotherapeutic utilization of half-bred saddle horses on chosen hematological parameters. The analyses included 10 half bred saddle horses, both sexes and of similar breeding parameters. There were not found any statistically significant differences as compared to the mean results concernings the sex. Red blood cell count was similar in all the samplings within the reference values. Regarding hemoglobin concentration and PCV in blood, the obtained results were within the limits considered as references. The differences between MCV values proved not to be statistically significant, they were found within the norm limits of all the samplings. Similarly, statistically significant differences were not reported for mean corpuscular hemoglobin per blood cell MCH. The MCHC levels were noted slightly below the reference limits. The leukocyte level and equine leukocytic image did not show any significant changes at the samplings and stayed within the reference values.